Here at MicroSolv, we are always investigating new and exciting
applications for our Cogent line of HPLC column products to address the
ever-expanding needs of today’s analytical chemists. I am particularly pleased
with these two latest application notes from our laboratories. Check them out,
as you may indeed find them interesting as well!
In the first one, we examined the selection of the
extraction solvent in influencing the extraction efficiency of ketorolac
pharmaceutical formulations. We observed that use of DI water led to superior
efficiency compared to ethanol. If you have encountered similar problems of low
recovery in your sample prep of pharmaceutical extracts, you may pick up a
pointer from the application. As for the chromatography, the compound was
well-retained in reversed phase using the Cogent Phenyl Hydride™ column:
The second application is for sucralose, commonly known by
its brand name of Splenda®. In addition to being significantly sweeter than
ordinary table sugar, sucralose has the further advantage of not contributing
to elevated blood sugar levels among diabetics. If you’ve analyzed sucralose in
your laboratory, you may have had problems with obtaining adequate sensitivity.
UV absorption is low in this compound but it can be detected at sufficiently
high concentrations and low wavelengths. Alternatively, you can try a universal
detection method such as refractive index (RI). We tried both approaches in our
application:
I am looking forward to seeing what unique applications will
be devised with these columns next!
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