In a recent article
published in the Journal of Chromatography A, Cífková et al. have investigated the chromatographic behavior of various
phospholipids such as (lyso)phosphatidic acids and (lyso)phosphatidylserines. If
you have ever done these kinds of compounds then you know they present a
challenge to say the least; they contain polar functional groups which make
them less suitable for chromatographic methods used to retain more hydrophobic
lipids.
Furthermore, identification of discreet lipid classes is
greatly facilitated by the high specificity of LC-MS, but previous methods
reported in the literature tend to use ion pair reagents, which are not
amenable to MS. This can be painful.
The authors present a comparison of columns for the LC-MS
separation of these compounds in real world samples (porcine brain and kidney
extracts). In terms of peak symmetry, the Cogent Diamond Hydride™ produced
tailing factors in the range 1.0–1.6 for all the studied analytes. Two analytes
in particular tailed so severely on HILIC columns that a tailing factor could
not be calculated; in contrast, the same two compounds produced only moderate
tailing (1.4 and 1.6) on the Diamond Hydride™.
If you struggle with phospholipid analysis, this paper is
well worthwhile reading as it is full of new insights.
REFERENCE:
E. Cífková, R. Hájek, M. Lísa, M. Holčapek, Hydrophilic
interaction liquid chromatography–mass spectrometry of (lyso)phosphatidic
acids, (lyso)phosphatidylserines and other lipid classes, J. Chromatogr. A
(2016), in press.
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