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Friday, October 4, 2013

Visit our Poster Sessions at Eastern Analytical Symposium

This year MicroSolv will be presenting two technical posters at Eastern Analytical Symposium in Somerset, NJ. The presentations will be held in the “Applications of HPLC and UHPLC” segment on Wednesday November 20, 2013 from 12pm–2pm.  Please stop by our booths and learn how your laboratory can benefit from these exciting new technologies.

                The first presentation is entitled “Advantages of Reduced Surface Activity (RSA) Glass Autosampler Vials for Basic Solutes.” In this study, our acclaimed RSA™ autosampler vials were compared with those of a market leading manufacturer based on conventional borosilicate glass. A variety of test solutes were assayed at different time intervals and concentrations using both vial types. The results of the study led to a number of interesting conclusions. First, only solutes containing basic functional groups showed any loss in peak area after a given time interval had elapsed, suggesting that interaction with silanols on the glass surface was responsible for the change. Second, the peak area loss was found to be drastically lower when using the RSA vials vs. the ordinary vials (see Fig. 1), which is believed to be due to the mostly silanol-free surface of the former. Third, the effect was more significant at early time intervals and leveled off after about 4 hours. This effect could lead to problems with precision as well as accuracy if different peak areas were obtained for consecutive injections in an analysis.

                In the second presentation “Separation of 1,3-Dimethylamylamine and Other Polar Compounds in Dietary Supplement Formulation Using Aqueous Normal Phase Chromatography with Mass  Spectrometry,” we use the Diamond Hydride™ HPLC column to separate hard to retain analytes. In a workout supplement called Jack3d®, each polar compound is retained and separated without the use of ion pair agents, making the method suitable for LC-MS. One ingredient (1,3-Dimethylamylamine) is controversial in terms of safety for consumption and has already been banned in a number of countries. As more data is accumulated for demonstrating the potential hazard of this compound, a need will arise for accurate and reliable quantitation in a variety of matrices. Therefore the Diamond Hydride™ column can play a significant role in these analyses.

                A technical program of the poster sessions is available here. We look forward to seeing you at the symposium this year!

 


Figure 1. Percent recovery of 5.00 ppm cetylpyridinium chloride solution at time intervals of 1 hour each for 4 hours. Both vial types are compared.

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