Green Chemistry Ideas in HPLC: Use Acetone

I have found that in terms of green chemistry principles, acetone is a superior HPLC mobile phase solvent than acetonitrile since it is significantly less toxic to humans and the environment. Another green advantage of acetone is that it is easier to recover and reuse than acetonitrile.

However, we all know that acetone has notable drawbacks which generally have precluded its use in routine HPLC methods over the years. It has significant absorption in the UV range and therefore is generally unsuitable for UV-based detection methods. Also increased tailing, broader peak widths, and lower retention have been observed for studied peptide analytes compared to acetonitrile when using conventional reversed phase HPLC columns. I am happy to say that I have used the Cogent Diamond Hydride™ column and very similar chromatography was obtained for most of the seventeen amino acids.

We investigated Acetone use with LC-MS and varied isocratic conditions (see Figure for comparisons). These findings suggest that acetone can be used interchangeably with acetonitrile in many instances when using the Cogent TYPE-C Silica™ columns.

For more information on the mobile phase solvent comparison study, see the following article in our online Knowledge Base or contact me:

http://kb.mtc-usa.com/article/AA-01017

 

Josh Young
MicroSolv Technology Corporation

Where have you gone Nicolaus Copernicus?

Making an impact on the human condition while seeking the truth, scientists have risked everything important to them in their life for their ideas. Even when the "field of experts" knew he was foolish to suggest such ideas, Nicolaus Copernicus published his findings and thoughts on our solar system. Of course, we know he was right and they were wrong. But what is most memorable and important is that he was not intimidated and continued to publish these ideas and helped move science and our understanding of nature forward. A true role model.

Today, we must ask ourselves, are we willing to face such feedback and criticism when we speak out for a better way or for better results? In our current bottom line oriented society it appears that so many scientists are intimidated into fast results instead of good results. Instead of truth, they are reduced to efficiency or data per dollar.

For the sake of science and our society, truth and innovation is the only way we will continue the proud and wonderful tradition that the men and women of science have established for us and that of which we are part. Discovery of truth in spite of "the field of experts".

We at MicroSolv salute and honor all scientists and we believe that without their work, the world would be more chaotic, difficult to survive and less livable.
Kelly, MicroSolv 2012

Who is MicroSolv Technology Corporation

Who is MicroSolv Technology Corporation?
At MicroSolv Technology Corporation we acquire and collaborate on new technologies for separation sciences. We design, produce, and distribute industry-leading products to chemistry, clinical, and biological laboratories worldwide.

Our expertise combines science, innovation and a culture of operational excellence to offer our customers technology services and support that help improve the quality of people's lives worldwide

Originating as a product line for Capillary Electrophoresis in 1992 MicroSolv was formally organized as a corporation in 1997. The company has evolved and grown and now we specialize in silica hydride based HPLC columns and Reduced Surface Activity autosampler vials and inserts. MicroSolv is best known for introducing and promoting ANP or Aqueous Normal Phase chromatography.

Mission Statement of MicroSolv Technology Corporation
Our mission is to encourage, develop, promote, assist, and aid in the improvement and development of chemistry and biological technologies. To participate in the discovery of new technologies that will improve the living condition of people and animals all over the world. It is within the scope of our mission that our customers using our technology will make a major improvement to the human condition. 

Also, our mission is to provide an easy, effective and efficient way to purvey information and to become an exchange of ideas that promotes science in both the professional and amateur fields.

Philosophy of MicroSolv Technology Corporation
MicroSolv Technology Corporation was founded on the principle of Quality. We therefore seek to provide quality in every aspect of our business. The minimum we will accept are quality products that perform and are easy to use and understand. Quality information provided to our customers, and other scientists, purchasing personnel and our vendors are integral to our philosophy. We also believe that our products, packaging and dealings should be as environmentally friendly as possible. We are always willing to listen to you for better ways to accomplish this.