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Monday, December 19, 2016

New Uses for Cogent™ Columns: Ketorolac and Sucralose


Here at MicroSolv, we are always investigating new and exciting applications for our Cogent line of HPLC column products to address the ever-expanding needs of today’s analytical chemists. I am particularly pleased with these two latest application notes from our laboratories. Check them out, as you may indeed find them interesting as well!

In the first one, we examined the selection of the extraction solvent in influencing the extraction efficiency of ketorolac pharmaceutical formulations. We observed that use of DI water led to superior efficiency compared to ethanol. If you have encountered similar problems of low recovery in your sample prep of pharmaceutical extracts, you may pick up a pointer from the application. As for the chromatography, the compound was well-retained in reversed phase using the Cogent Phenyl Hydride™ column:


The second application is for sucralose, commonly known by its brand name of Splenda®. In addition to being significantly sweeter than ordinary table sugar, sucralose has the further advantage of not contributing to elevated blood sugar levels among diabetics. If you’ve analyzed sucralose in your laboratory, you may have had problems with obtaining adequate sensitivity. UV absorption is low in this compound but it can be detected at sufficiently high concentrations and low wavelengths. Alternatively, you can try a universal detection method such as refractive index (RI). We tried both approaches in our application:


I am looking forward to seeing what unique applications will be devised with these columns next!